Essel Program - 2008 Annual Report


ANNUAL REPORT OF THE ESSEL NEUROSCIENCE PROGRAM AT WILLIAMS COLLEGE, 16th Year
July 2007 thru June 2008

The Neuroscience Program

The Program in Neuroscience had another strong and successful year.  Professor Noah Sandstrom received tenure.  Dr. Laurel Pritchard, the outgoing Senior Essel Fellow, started her new tenure-track position as Assistant Professor of Psychology at the University of Nevada, Las Vegas.  We are fortunate to have had Dr. Martha Marvin join us as the new role of Senior Essel Fellow; she had a busy and productive year with the program, teaching a section of the senior seminar as well as the lab program for our introductory course.  Our Junior Essel Fellow, Tracey Van Kempen, completed her term and will begin graduate work towards her Ph.D. in Neuroscience at Cornell in the fall.

This past year was also a successful one for the Program’s concentrators. In addition to the research assistants and independent study students conducting research with faculty members, there were thirteen Summer Essel Fellows in 2007.  All eight of the graduating Summer Essel Fellows (class of ’08) will be pursuing research in neuroscience as they enter the post-baccalaureate world – as research assistants, graduate students, or MD/PhD students.  John Rudoy, a former Essel Fellow and  thesis student of Noah Sandstrom’s, received a prestigious NSF Fellowship.

One highlight of the year was our students’ and program’s participation in the exchange of ideas within the wider neuroscience community. The program’s colloquium series, put together by Betty Zimmerberg (the outgoing Chair of the Neuroscience Program) was particularly strong and successful during the past academic year. Several students enjoyed the opportunity to attend NARSAD’s annual symposium in New York City.  Still others attended scientific conferences and some presented their own work.  Of particular note is that Kimberly Elicker was awarded the Undergraduate Student Poster Presentation Award by the American Association of Anatomists for her work on the “Characterization of the Zebrafish Small Heat Shock Protein Family”, a research project that she conducted under the supervision of Professor Lara Hutson.

On the Williams College campus, the program’s course offerings were popular.  Introduction to Neuroscience, the cornerstone of our program, was taught this year by Steve Zottoli and Noah Sandstrom and attracted nearly 100 students, and the senior seminar, the capstone of the program, needed to be offered as two sections to meet student demand.  We have also started outreach programs to local schools; Noah Sandstrom has given a very successful presentation on brains to the fourth graders at the Williamstown Elementary School, and all of the school’s sixth graders dissected sheep brains in the lab at Williams College in a program organized by Heather Williams.

Our faculty members continue not only to teach, publish scholarship, and supervise student research, but also to serve the wider college and scientific communities.  Betty Zimmerberg chaired both the Psychology Department and the Neuroscience Program, and Steve Zottoli chaired the Biology Department. Both Betty Zimmerberg and Heather Williams served on grant review panels for the N.I.H., and several faculty members received funding from the N.I.H. and other outside sources.  All have served as reviewers for scientific journals.

Williams College has begun a cycle of reviewing interdisciplinary programs, and Neuroscience was one of the first to undergo such a review.  This process included circulating petitions to past students, analyzing the responses, holding extended discussions, and writing a self-study report that answered specific questions raised by the review committee.  Betty Zimmerberg ably shepherded us through this process.  Not surprisingly, given the longevity and continued success of the program, the review was laudatory.  The primary question raised by the review committee was whether the Neuroscience Program should, because of its success and size, consider becoming an independent department with its own faculty and major.  At this time, we are not moving in that direction, but we will keep the possibility in mind for the future.

I. Undergraduate Research Fellows:

This past summer, thirteen students conducted research with the Neuroscience faculty.  Their research reflected the wide-ranging interests of the faculty with whom they worked.  For the eight Essel Summer Fellows graduating in 2008, summer research provided an invaluable experiential component of their education.  A summer research experience often inspires students to complete an Honors thesis, and five of the students in the class of 2008 who had previously been Essel Summer Fellows completed Honors research in Neuroscience this year. 

During the summer of 2007, an unusual number of students holding Essel Fellowships performed research off-campus (two of our faculty members underwent medical procedures and were not able to supervise students).  The off-campus fellowships proved to be valuable to the students, as can be seen by their accounts; although we will continue to emphasize research fellowships at Williams, we may also continue supporting off-campus summer research when and where it is appropriate.

The research topics and accomplishments of the fifteen summer research students are listed in Appendix I. The titles and abstracts of the seven Neuroscience theses that were completed in 2004 can be found in Appendix II.

II. Williams Neuroscience Faculty:

The faculty was actively engaged in research and teaching this past year. Faculty publications that were supported in part by Essel funds are listed in Appendix III and a summary of all of their work in addition to publications can be found in Appendix IV. Appendix V enumerates all other current sources of funding for Neuroscience faculty and the corresponding project.

III. Neuroscience Courses:

In addition to the introductory neuroscience course (NSCI 201) and the capstone course (NSCI 401), we offered five elective courses this year; two of the electives required the students to design and perform their own research project, and all of the elective courses gave students the opportunity to gain research experience.  A complete listing and description of courses, including the syllabi of the two new courses, is located in Appendix VIII.

IV. Essel Fellows:

This was Dr. Martha Marvin’s first year as the Senior Essel Fellow in Neuroscience.  Her teaching duties included the laboratory sections of foundation course for the program (Introduction to Neuroscience) and the spring section of the senior seminar (“Birth, Death and Stem Cells in the Adult Brain”).  Her research program coordinated with that of Lara Hutson, and focused on the role of small heat shock proteins (sHSPs) in zebrafish development. Dr. Marvin’s work this year concluded a survey of the expression patterns of sHSPs over the first 48 hours of development (Marvin et al, 2008).  At present, she is studying the effect of reducing expression of Hsp27 in early development.  Loss of Hsp27 affects the early patterning of the nervous system, causing a reduction of posterior nervous system tissue.  This patterning defect suggests that Hsp27 is necessary for one or more cellular signaling pathways essential to normal development. She presented these findings at the Zebrafish Development and Genetics Meeting.  Such a role for small heat shock proteins would be a novel finding and would add significantly to our understanding of the protective effects of these proteins in development and stress.  This summer Dr. Marvin is working with two Williams students, Christina Liu and Hannah Rosenthal, on her ongoing research project - identifying the pathways affected by reduction of Hsp27. 

Tracey Van Kempen completed her third year as the Junior Essel Fellow. She assisted in the teaching of the laboratory component of the Introductory Neuroscience course and served as the teaching assistant in Dr. Zottoli’s upper level Neurobiology course. Continuing her research with Betty Zimmerberg, Ms. Van Kempen had the opportunity to present her work at the Annual Meeting of the Society for Neuroscience in San Diego. During January, she had the opportunity to teach a Winter Study course exploring how animals and humans communicate and build relationships with each other. “Animal Communication: The Psychology of Human-Animal Relationships” examined the psychological and neurobiological principles of behavior, communication, and social bonding. Field trips to the local humane society, horse training facility, and a visit with an equine veterinarian provided experiential context for the students. In the fall, Ms. Van Kempen will be attending the Weill Cornell Graduate School of Medical Sciences pursuing a PhD in Neuroscience.

V. Colloquium Speakers:

The Neuroscience program had continued success in bringing interesting and qualified speakers to campus this year. A listing of these speakers and their topics and affiliations can be found in Appendix VI.

VII. Budget:

Appendix VII presents the expenditures for year 11. An explanation of expenditures is also provided in that appendix.

 


APPENDIX I: 2007 ESSEL NEUROSCIENCE FELLOWS

Student:

Shannon Chiu

Advisor:

Solomon

Project:

Examining the efficacy of cognitive exercise in Nintendo’s ‘Brain Age’

Currently:

Working in a related field

 

Having read about myriad case studies in my clinical neuroscience class this spring, I was thrilled to have the opportunity to work in Professor Paul Solomon’s Memory Clinic as a student intern.  Not only did I get a chance to meet his patients firsthand – many of whom suffer from Alzheimer’s and other disease-driven dementias – I was given entrée to the world of neuropsychology that uniquely combines clinical and research work.
As a start, I learned to administer various neuropsychological tests on clinic patients.  Neuropsych examinations are important diagnostic tools for determining the existence of brain injury or disease.  They are objective tests that specifically explore brain function, from verbal fluency, memory and language, to visiospatial skills, motor function and more.
A major part of my summer experience was participation in the Brain Age study, which examined the efficacy of a new Nintendo program that features games and puzzles (e.g. Stroop tests, math problems, counting currency, Sudoku puzzles and other brainteasers) to stimulate the brain.  According to the official distributor of Brain Age, neurological studies using functional magnetic resonance imaging (fMRI) have shown that processes of reading aloud and practicing mental math may effectively train the brain.  In short, the Brain Age game is built on the basis that routine cognitive exercise improves blood flow to the brain, which in turn improves executive functions in the prefrontal cortex.
My lab partner and I recruited cognitively healthy adults (age = 40+) from Berkshire County.  Subjects were given a full neuropsych evaluation during their baseline visit.  Afterwards, they were randomized to follow the experimental or control protocol for one month.  In the former, subjects were instructed to play the Brain Age game daily for 30 minutes; in the latter, they were given only word-search puzzles, also to be completed daily for 30 minutes.  After one month, subjects returned for their second full neuropsych evaluation.  In both visits, tests comprised of verbal and visual memory (immediate and delayed); digit and symbol recall; serial addition; color-word interference; digit span; trail making; word learning; and Wisconsin card sorting.
In spite of the well thought-out parameters and cooperation of the test volunteers who were so generous with their time and patience, it was difficult to gather enough data given the timeframe of the summer.  Moreover, due to limited resources in testing human subjects, objective testing of changes in brain function, or lack thereof, is restricted to neuropsych testing.  It would be more relevant to be able to compare patients’ brain waves or activation levels during testing.  After all, EEG recordings or brain scans could provide a more accurate and biologically compelling picture of the game’s effects on brain function.
Still, over the course of my internship, I derived much satisfaction from what I did in and out of the clinic.  While the neuropsych battery seemed a little repetitive at times, I was always intrigued to meet a new patient during each assessment.  Be it healthy subjects for the Brain Age project or clinic patients, I was included in the lifecycle of another, albeit on the periphery; and I was doing my tiny part in smoothing out existing or potential rough patches in their lives.  However, most of all, my time in the clinic was a revelation for someone considering a career in the medical sciences.  I was able to witness variable manifestations of neurodegenerative diseases such as Alzheimer’s.  Memory loss aside (one of the most commonly known symptoms in AD), I was struck by patients’ spontaneous and inexplicable emotional breakdowns; their disconnection from the world around them; their loss of identity.  They are robbed of the ability to communicate and maintain interpersonal relationships – both of which are fundamental human motivations.  It made me realize, too, the destructive effects of such diseases inflicted upon patients’ loved ones.  The emotional burden and exhaustion are not merely caused by patients’ increasing dependency, but more importantly, by the fact that they become complete strangers to families and friends.  Every patient’s story is a call for help and with the rapid aging of our nation, a breakthrough in prevention and treatment is desperately needed.  I see myself playing a role in this vast effort and will take my first steps by continuing work at the Memory Clinic and on the Brain Age research project, as well as working in a related field after graduation.

 

Student:

Gordon Crabtree

Advisor:

Sandstrom

Project:

Estradiol-mediated neuroprotection in the hippocampus

Currently:

Working as a research assistant prior to applying to medical school

 

Over the summer, I worked on a project similar to my thesis that had to do with targeted estradiol-mediated neuroprotection in the hippocampus.  My main duties for this project included learning and performing surgeries on rats and some basic histology.  I was also involved in maintaining the inventory of rats, including the rate of rat breeding.
The summer was a great time for me to really hone my surgical and other lab skills.  The one weakness of the summer was that I did not have much instruction toward the end of my time here; however, this ended up being one of the summer's greatest strengths.  The time without immediate supervision forced me to work around problems that arose in the lab and to take the initiative on planning my schedule.  This allowed me to become more confident in the surgeries and also to develop a more focused plan of attack for the ensuing school year.

 

Student:

Colleen Farrell

Advisor:

Hutson

Project:

Charcot-Marie-Tooth Disease

Currently:

Junior Chemistry major

 

This past summer I worked in a neurobiology lab with Professor Lara Hutson.  The lab investigated Charcot-Marie-Tooth Disease by creating mutant strains of zebrafish and examining their axons.  I learned many different biological techniques and used different types of microscopes.  It was a great experience to meet other students and faculty.  Though I have decided I do not want to pursue a career in research, this experience helped me learn that about myself.  I think the lab was maybe not a good fit for me because I am a chemistry major and at the time had taken only one biology course. 

 

 

Student:

Jonathan Horn

Advisor:

Solomon

Project:

The Cognitive Effects of Anti-Dementia Compounds

Currently:

In the process of applying to medical school.

 

This past summer I worked on several different projects with Professor Solomon.  My main focus was on the effects of anti-dementia compounds.  I spent much of the summer performing behavioral and memory testing to evaluate the cognitive effects of anti-dementia compounds used by patients involved in several different clinical research projects.  In order to compile and organize the data collected during these projects, I worked with Professor Solomon to develop a database to allow us to track the neuropsychological test data.  I really enjoyed my summer spent doing cognitive testing and research.   Spending a summer working with Professor Solomon provided me with a valuable opportunity, which contributed to my decision to apply to medical school.

 

 

 

Student:

Natashia Kadimik

Advisor:

Sandstrom

Project:

Neuroprotective Effects of Estrodial Following Transient Global Ischemia

 

In the lab, I performed ovariectomies, four-vein occlusions, and perfusions on adult female rats.  I also sliced and stained brain tissue, performed behavioral testing, and collected data for research projects supervised by Professor Sandstrom and Gordon Crabtree (who wrote a thesis with Prof. Sandstrom).  It was great to be in Williamstown over the summer, and to have so much time to devote to a specific project.  It's impossible to get that kind of experience during the school year with so much else going on.  Apart from the experience of being at Williams for my last summer as a student, I also found the experience a fantastic opportunity to become very close to Prof. Sandstrom and to Gordon, and to learn more than I could have even imagined about estradiol, 4VO's, and rats in general.  The summer also led to a position working in the lab for the following school year, which was a wonderful opportunity.

 

Student:

Michael Kirwan

Advisor:

Zimmerberg

Project:

Behavioral task performance and cell receptor densities underlying maternal behavior in three different strains of NIH rats bred for infantile ultrasonic vocalizations

Currently:

Graduate student in the Neuroscience and Cognitive Science (NACS) PhD program at the University of Maryland - College Park.  Mike will be working through the Human Development department under Williams alumnus Nathan Fox '70.

 

The project I worked on at the New York State Psychiatric Institute (NYSPI) last summer had me measuring both behavioral task performance and cell receptor densities underlying maternal behavior in three different strains of NIH rats.  Originally, the three lines were selectively bred to display either high, random, or low levels of USV as pups, and studies since the creation of the lines have shown additional constellations of traits that correspond to each line.  One such trait is the absence of play behavior in the low line juveniles, a social deficit that could potentially be a model for some autistic social behaviors.  The low line males also display more frequent bouts of aggressive behavior as adults when cohabitated with other males, potentially due to an inability to defuse novel social situations.
I studied dams from all three lines, observing maternal behavior on postnatal days two, four, and six.  I specifically looked for differences in nest quality, time spent in nest, time spent nursing, frequency of arch-backed nursing and milk letdown, and frequency of anogenital (AG) licking.  Animals whose family lines had more than enough females to continue breeding were then sacrificed on day six, perfused, and the brains were removed to be sectioned and stained.  Brain sections of the ventral tegmental area of the hypothalamus, the nucleus accumbens, and the prefrontal cortex were double labeled for either oxytocin or vasopressin in addition to cfos.  Density of both vasopressin and oxytocin receptors was then correlated with frequency of maternal behaviors during nursing bouts.
While the experiment was not finished when I left, I did learn a great deal from the experience.  My only previous lab experience in neuroscience was through the Hormones & Behavior class at Williams, and my experimental techniques there were limited to ovariectomies, castrations, silastic capsule implantations, and the Barnes maze cognitive measure.  Through this summer’s program, I learned to perform perfusions, remove and section brains, and double label the sections with receptor-specific antibodies.  The only weakness I can see in the entire experience was the slow stream of animals with which I could work.  Due to space limitations, the colony was confined to one large room housing all three lines of rats, and frequency of breeding was limited by the space of that room.  While this regrettably kept me from gathering all the data desired to complete the project and move on to data analysis, I did manage to complete a large portion of the research and learn several new lab techniques in the process.

 

Student:

Nicholas Manice

Advisor:

Hutson

Project:

The Characterization of Heat Shock Proteins in dHMN and CMT in Zebrafish

Currently:

Senior biology major at Williams.

 

We attempted to recreate the mutations that cause CMT and dHMN in zebrafish so that we could determine the effects of environmental stressors on CMT.  I learned to run lab experiments such as minipreps, site directed mutagenesis, PCR reactions, and RNA preps. The bulk of my time was spent doing microinjections on zebrafish embryos.  The strengths of the program are that it builds relationships between the faculty and the students.  It is often difficult to bond with a faculty member during the year.  This summer program helped me meet some fantastic professors.  One weakness is that different labs tend to remain very separate from each other and it would be great if there were more functions to bring us all together.  Overall, I had a fantastic time last summer getting to know several faculty members and participate in research that I believe will actually benefit many people some day.  I am very grateful for this opportunity.

 

 

 

Student:

Ashley Rae Martinez

Advisor:

Zimmerberg

Project:

Spatial learning in aged rats bred for high and low levels of infantile ultrasonic vocalizations

Currently:

Senior Neuroscience concentrator and Biology major

 

I would describe my summer research experience as being immensely beneficial and enjoyable.  Conducting my own experiment gave me an opportunity to learn and execute organizational and planning techniques that assisting with projects developed by others did not allow for.  One thing I wished that I had done differently was to begin testing rats on the Morris water maze earlier so that I would have had more time for data analysis.  Doing this would have allowed me to master data analysis and better speculate about potential explanations for my results.  Getting an earlier start on testing might even have made it possible to repeat or further examine significant results either on different mazes or with different manipulations.
     In addition to working on my research project, I assisted with finishing a thesis student’s work.  I enjoyed working on two projects at the same time, with the different projects each demanding the mastery of different lab skills.  Working on several projects at once also provided a diversity of procedures that prevented one project becoming too monotonous or consuming.  Having several projects going on in lab also helped me improve my time management and organizational skills, making me constantly aware of when things needed to be done.
Working with Lindsay Moore was truly a fantastic lab experience.  Together we were able to correct one another’s oversights and construct solutions that one of us alone might not be able to conceive.  Dividing the work between ourselves in a way that ensured time efficiency and our own efficiency in conducting certain tests and executing specific procedures was a summer long effort that undoubtedly enhanced my ability and eagerness to work in lab with others.
The biology lunches were a definite highlight throughout the summer.  They provided a nice break from research and opportunity to meet and interact with other Williams research students.  I greatly enjoyed the talks, in which I was able to learn and ask questions about other students’ and professors’ research. 

 

Student: 

Lindsay Moore

Advisor:

Zimmerberg

Project:

The Effects of Maternal Separation on Olfactory Association Learning and BDNF in the Neonatal Hippocampus and Olfactory Bulb

Currently:

Senior Neuroscience concentrator and Psychology major

 

This summer, I worked in Professor Betty Zimmerberg’s psychology lab.  My research primarily focused on compiling data and performing follow-up testing for a graduated senior’s thesis to better interpret her results.  The experiment involved analyzing BDNF (brain-derived neurotrophic factor) protein levels through a series of assays and treatments in both the hippocampus and olfactory bulbs in infant pups at post-natal day seven; conditions and controls were assigned, including variations of pups that were separated from their mothers for an extended period while also experiencing odor/milk conditioning and our control group of pups that were undisturbed entirely.  In addition, we tested the certain groups of pups’ behavioral preferences for either odor or bedding scent.
     Working in Professor Zimmerberg’s laboratory for the summer was absolutely amazing!  I had never had the opportunity to conduct independent research before, and this experience has already proven incredibly valuable.  It taught me the details of conducting one’s own experiment and the mentality that is needed to succeed in the science research arena.  Strengths of the program included the autonomy I was given to really experience life as a researcher, while being given significant (and much appreciated/needed) support by Junior Essel Fellow, Tracey van Kempen, and Professor Zimmerberg.  Tracey’s constant guidance in the laboratory was probably the most important factor in my learning and growth over the summer; having a former student able and willing to help every step of the way made learning the protocol less daunting.  I also feel that allowing undergraduate students to conduct research is essential for developing students’ abilities for graduate degree programs.  It is a rare privilege at an undergraduate level and one that is deeply appreciated.

 

Student:

Nicole Sanders

Advisor:

Zimmerberg

Project:

The effects of alcohol consumption and withdrawal on sensitivity to alcohol-induced hypothermia

Currently:

Attending medical school after continuing her research at UCLA during the summer

 

I worked at the Neurobiology Brain Research Institute at David Geffen School of Medicine at UCLA with Dr. Anna Taylor.  I assisted in two of her studies.  The first was "The Effects of Alcohol Consumption and Withdrawal on Sensitivity to Alcohol- Induced Hypothermia".  In this study, I assisted in alcohol administrations, tail bleeds, and blood alcohol concentration analysis of rats.  I was also responsible for some literature review, performed data analysis, and provided summary of data.  The second study was a Traumatic Brain Injury study.
The data for this study was done prior to my arrival.  However, I photographed hippocampal cell loss and analyzed cortical and hippocampal cell loss from the slides produced earlier in the year.  From this data, I performed data analysis and provided a summary of my results that were used in a recent publication of this study, “Injury Severity Differentially Affects Short- and Long-Term Neuroendocrine Outcomes of Traumatic Brain Injury” published in the Journal of Neurotrauma April 2008.  Near the end of the summer, Dr. Taylor repeated the TBI study in which I was able to assist in traumatic brain injury surgeries and in administering a forelimb task on the rats.  The summer research experience was very informational on how medical research is conducted.  I was able to acquire insight into whether or not I want to pursue research in the future as a career.  However, the bad thing about summer research is that you usually enter a lab in the middle of studies, which makes it difficult for the student because you initially feel as though you are impeding the research.  The PI has to take time to teach you the procedures or methods used and inform you about the study.  In my case, working on two studies simultaneously was very time consuming for a three-month period.  Ultimately, I had fun.

 

Student:

Charles Upton

Advisor:

Williams

Project:

Thermoregulation and Invasion in Polistes

Currently:

Working as research assistant

 

First off, I want to thank the Williams Neuroscience Program and the Essel Foundation for the opportunity to do research off campus this summer.  I spent the summer working at Tufts University in the lab of Dr. Philip T. B. Starks.  Dr. Starks studies behavior in honeybees (Apis mellifera) and paper wasps (Polistes sp.).  My particular project, entitled “Thermoregulation and Invasion in Polistes” dealt with flight behavior and thermoregulation in two local paper wasp species, one native (Polistes fuscatus) and one invasive (Polistes dominulus).  The question I explored asked whether differences in thermoregulatory ability during flight were affecting the invasion event.  I did the bulk of the research, first demonstrating that the wasps were thermo-regulating (never been done before!) and measuring their flight temperatures.  There is ongoing research being replicated from my methods.
The program allowed me to branch out into different fields of science outside of the animal behavior I was originally interested in, which I felt was great experience.  I was also required to present my research in a symposium at Tufts, something I had never had to do in a formal situation, and I was asked to write a methods section for the paper that will be sent to be published as a result of this research.  I also got a lot of great advice regarding graduate school from both students and faculty at Tufts.
The Essel student program was excellent for increasing my experience and interest in behavioral research as well as allowing me the freedom to choose where I worked. Thanks again to the Essel Foundation for affording me this opportunity. 

 

Student:

Erika Williams

Advisor:

Sandstrom

Project:

Development of an assay to detect adenosine release from glia, astrocyte, and neuron cultures in response to metabolic stress

Currently:

Field assistant studying capuchin monkeys in Lomas Barbudal, Costa Rica while applying for MD/PhD programs for matriculation in the fall of 2009.

 

At Rochester, I worked on developing an assay to detect adenosine release from C6 glioma, astrocyte and neuron cultures in response to metabolic stress and oxygen glucose deprivation.  Adenosine is an inhibitory neuromodulator and potent neuroprotectant, the concentrations of which increase 100-fold in the brain during ischemia.  I wanted to determine the source, the mechanism of release and whether other neuroprotectants such as estradiol can potentially act by affecting or enhancing adenosine release.  I was able to optimize an effective assay for adenosine samples, but was unable to get the assay to function for samples drawn from cell cultures.  I think this had to do with free radical scavengers produced by cells interfering with the hydrogen peroxide created by and necessary for the successful assay.  Given the failure of this assay, toward the end of the summer I began working on another project at Williams to get at the question of mechanisms of neuroprotection during ischemia.  More specifically, I began characterizing a model of global ischemia in mice.  I wanted to do this to get access to genetic technologies used in mice (such as knockouts) so that I could determine the receptor subtypes involved in neuroprotection conferred by the hormone estradiol following this form of neural insult.  This question interested me because estradiol itself is a potent neuroprotectant, but not a viable therapeutic agent because of its non-specific action throughout the body.  Therefore, more targeted treatments must be developed, and knowledge of mechanisms of estradiol protection could guide this development.  This later work became the basis for my senior honors thesis project.

 

 

 

Student:

Tina Wong

Advisor:

Zottoli

Project:

Receptive field of supramedullary neurons in cunner

Currently:

Student in the MD-PhD at the Mayo Clinic College of Medicine

 

I performed electrophysiological studies examining the receptive field of supramedullary neurons by giving either electrical or touch stimuli to the fish's body and recording the responses of these cells to the stimuli intracellularly.
For the second consecutive summer, I came to Woods Hole, MA to do research at the Marine Biological Laboratory.  Even though the diverse scientific community here can sometimes pull me away from my own research, the opportunities to attend evening lectures and learn about fields outside of my own study were endless.  Especially as a young scientist, this experience has allowed me to explore science in ways that I could not otherwise.



APPENDIX II: ABSTRACTS OF NEUROSCIENCE HONORS THESES

The Neuroprotective Effects of Acute Hippocampal Estradiol Injection in Ischemia
Gordon S. Crabtree

Advisor: Noah Sandstrom

Transient global ischemia is an event that causes a loss of blood flow to the brain, resulting in severe behavioral, spatial learning and memory  and anatomical, cell death  damage.  This study uses a rat 4-VO model to create a transient global ischemia, and investigates the ability of an acute injection of estradiol directly into the hippocampus to lessen the damage associated with the ischemia.  It also explores the mechanism that estradiol uses to be protective.  Rats were given an acute injection of either estradiol or a vehicle immediately after the 4-VO and outcomes were based on performance on the c-MWM and h-MWM.  The results suggest that estradiol is effective in mitigating the effects of the ischemia if administered directly into the hippocampus, suggesting that  estradiol works in the hippocampus, and not in other, more remote, regions of the brain.

 

Note Order Variability in Directed Song Sequences:   A Heritable Trait?
Mildred Duvet

Advisor: Heather Williams

Male zebra finches (Taenopygia guttata) sing consistent and stereotyped songs.  Of their two types of songs, directed song (courtship song), sung to females, is more stereotyped, linear and consistent and is less variable in syllable structure than undirected song, sung alone or in the presence of other males.  However, variability in note order was observed in the directed songs of a population of adult male zebra finches.  In a previous study, birds with high and low note order variability were bred selectively for one generation (F2), and preliminary results indicated that the degree of note order variability may have been a heritable trait as suggested by Hunter (2003).
In this study, I continued a selective breeding program initiated by Hunter (2003) and bred a third generation (F3) of male zebra finches selected for low and highly variability in the sequence of their directed songs in order to determine whether this trait is heritable.  Testis weight and testosterone were measured to determine if these characteristics were related to note order variability.  The volumes of the “heritable song nuclei,” HVc, RA and LMAN, were also measured to determine if they were related to syllable order variability in directed song.
This study did not find evidence supporting the hypothesis that syllable order variability is a heritable trait.  The F3 generation did not resemble their fathers in variability scores.  The volumes of HVc and RA were not correlated to note order variability, sequence consistency, linearity nor stereotypy.  There was a correlation between LMAN volume and sequence linearity, and the relationship between LMAN volume and note order variability and sequence stereotypy approached significance.  However, this relationship was driven mostly by an outlier and LMAN volume was not significantly correlated to sequence consistency.  Further, there was no significant correlation between the weight of the testes or the level of testosterone in the blood and note order variability, linearity, consistency and stereotypy.  Thus my results suggest that other environmental factors or possibly the complicating effects of the non-singing females genetic input may be responsible for syllable variability in the directed songs of male zebra finches.

 

The Role of Torpor in Memory Consolidation in Mice
Sarah G. Nowakowski

Advisors: Steve Swoap & Noah Sandstrom

Memory consolidation is the process by which new and labile information is stabilized as long-term memory. The consolidation of memories involves the formation of new cortico-cortical synapses, which is thought to be mediated by the hippocampus; and it is the formation of these new neuronal connections that solidifies newly learned information, thereby preventing degradation. This research analyzes the role of torpor, a hypometabolic state experienced by small rodents that is induced by cold ambient temperatures and low food availability, in the process of memory consolidation. Animals were trained on the Morris Water Maze (MWM) Hidden Platform task, and subsequently exposed to one of four experimental manipulations for 23 hours. Animals were either fasted at 19°C, fed at 19°C, fasted at 29°C, or fed at 29°C. All of the animals that were fasted at 19°C entered torpor while none of the animals in the other conditions experienced a torpor bout. Twenty-three hours after the initiation of the experimental manipulation, all animals were tested on the MWM in order to assess the consolidation of the memories previously learned. When tested 23 hours after learning, animals that were fed at 19°C and those that were fasted at 29°C did not demonstrate a bias toward the target platform, suggesting the existence of a disruption in the consolidation of newly acquired information. Animals that were fasted at 19°C, and therefore entered torpor, and animals that were fed at 29°C were capable of recalling the spatial memories learned during acquisition. These data demonstrate that the physiological changes that occur during torpor result in functional protection of the memory consolidation that is involved in hippocampally-dependent learning of spatial navigation tasks.


Characterization of Points of Variability in Zebra Finch Song
Charles T. Upton

Advisor: Heather Williams

Song is a sexually dimorphic characteristic of the zebra finch (Taeniopygia guttata), an oscine songbird native to Australia and Timor.  Song is used as a courtship strategy by males and is a learned trait (Immelmann 1969).  Song is passed on through generations to listening juveniles, who, through a series of vocal imitation processes, construct their own song.  However, in other oscine bird species, song learning is mediated by innate processes as well as by learned imitation (Gardner et al. 2005).   
Male zebra finches undergo a song crystallization event marking the onset of sexual maturity.  After this crystallization event, the bird will sing the same motif in its songs for the rest of its life (Immelmann 1969).  However, an adult zebra finch’s song does have a certain degree of variability within each motif.  This thesis concerns structural variation, or differences in note order, rather than the variability of the notes themselves.  These instances of sequence variability can include note deletions, repetitions, and insertions.  Each instance of variability can be associated with a point in the motif at which the note sequence differs from the individual’s canonical, or most common, motif. 
To characterize these points of variability between notes, including the prevalence of the instance of variability I have assessed a number of song features including the position of the point of variability in the motif, the duration of the interval between notes, and the type of note before and after the interval.  My goal is to find trends in these points of variability based on analysis of these features.  I have found that similar note structure does not imply similar trends in variability.  Birds with very similar songs displayed divergent patterns of motif variation.  In addition, certain syllable chunks which are consistent through several birds’ motifs are rarely interrupted by instances of variability.  I observed other specific trends, such as the tendency to delete stack notes from the end of a motif.  I used visible trends such as this as testable hypotheses.  My analysis supports evidence for several consistent trends across individuals, including points of variability early in the motif and surrounded by a specific combination of bounding notes being associated with high variation prevalence.  The fact that such trends exist suggests that, despite variation between individuals with nearly identical songs, there are inherent aspects of song that affect sequence variability.


Development and Characterization of a Mouse Model of Transient Global Ischemia:
Mechanisms of Neuroprotection by Estradiol
Erika K. Williams

Advisor: Noah Sandstrom

Strokes and related ischemic events are one of the top three most common causes of mortality in developed countries. Given the prevalence and costs of stroke, ability to combat negative stroke outcomes would be a substantial contribution to healthcare. A compound of particular interest in this context is the hormone estradiol since it is protective against many forms of neurodegeneration, injury and insult, including several types of stroke. However, while estradiol acts as a neuroprotective agent, there are legitimate clinical concerns regarding its use because of a variety of adverse side effects associated with its use. Therefore, it is important to understand the mechanism through which estradiol is protective so that more targeted treatments can be developed. The most convincing explanation for estradiol protection is action through estradiol receptors, ERs. There are two classic ER subtypes, a and b and the involvement of each subtype in protection remains unclear. The current study characterizes a model of transient global ischemia in mice and evaluates the modulatory role of estradiol. Two-vessel occlusion, 2-VO is a model of transient global ischemia that involves reduction of blood flow to the brain via clamping of the common carotid arteries. Inability of ink injected into the left ventricle of the heart to reach the forebrain provided evidence that this is a feasible and reproducible stroke model. 2-VO caused region-specific cell death in the cortex, caudate and the hippocampus as measured with fluorojadeB, FJB, a marker for cell death. Longer durations of 2-VO also resulted in more severe mortality and more extensive damage in the hippocampal cell body layers. The interaction between region and ischemia duration was also significant, suggesting that different regions exhibit different susceptibilities to ischemic insult. The time course of expression of our marker for cell death, FluoroJade B, FJB, indicated that the number of FJB+ cells decreased marginally as soon as one week after insult throughout the hippocampal layers. Implantation of estradiol capsules prior to 2-VO reduced cell death selectively in the CA1 cell body layer. In sum, 2-VO is a suitable stroke model and estradiol reduces damage associated with 2-VO. These studies establish and characterize a model of transient global ischemia in mice, a particularly powerful development because it allows access to genetic technologies available only with mice that can be used to further probe the cellular underpinnings of this protection.


A Study of Electrphysiological Properties and Function of Supramedullary Cels (SMC) in the Green-Spotted Puffer, Tetraodon nigroviridis
Tina Wong

Advisor: Steve Zottoli
Supramedullary neurons (SMC) are large cells located in a cluster on the dorsal surface of the medulla oblongata of pufferfish. These cells have outputs that end in association with subepidermal mucous glands. The function of SMCs is not known, but to date the most favored hypothesis is that these cells stimulate mucous secretion upon activation. To better elucidate the role of these neurons and test the validity of this hypothesis, we have characterized the anatomical and physiological properties of these cells in the green-spotted puffer. Furthermore, we recorded activity from the cluster of SMCs in a free-swimming animal as an attempt to correlate brain activity with behavior. We have found that each SMC responds repetitively to synaptic input from the entire head and body of the animal and that SMCs are spontaneously active in both restrained/immobilized and in free-swimming pufferfish. Our results are consistent with the hypothesis that SMCs play a role in the activation of mucous secretion.


APPENDIX III: ESSEL SUPPORTED PUBLICATIONS AND PRESENTATIONS

* indicates student author

Lara Hutson


Elicker, KS* and Hutson, LD (2007) Genome-wide analysis and expression profiling of the zebrafish small heat shock proteins.  Gene, 403, 60-9.

Marvin, MJ, O’Rourke, D*, Kurihara T*, Juliano, CJ*, Harrison, KL*, and Hutson, LD (2008) Developmental expression patterns of the zebrafish small heat shock proteins.  Dev Dyn,  237, 454-63.

Martha Marvin

Marvin, MJ, O’Rourke, D*, Kurihara T*, Juliano, CJ*, Harrison, KL*, and Hutson, LD (2008) Developmental expression patterns of the zebrafish small heat shock proteins.  Dev. Dyn.,  237, 454-63.


Noah Sandstrom

Sandstrom, N. J. (in press).  Sex differences in use of visual cues by rhesus monkeys performing a spatial learning task.  Comment on "Cognitive performance in rhesus monkeys varies by sex and prenatal androgen exposure" by Herman and Wallen.  Hormones and Behavior, 52, 139-142.

Rowan, M. H.*, Williams, E.K.*, Sandstrom, N. J. (November, 2007).  Neuroprotective effects of acute estradiol depend on the time of administration and the age of the rat.  Society for Neuroscience Conference, San Diego, CA.

Chuzi, S.E.*, Williamson, L.L.*, Crabtree, G.S.*, Cavigelli, S.A., Sandstrom, N. J. (June, 2007).  Early life exploratory behavior predicts anxiety and corticosterone stress response, but not spatial learning, in adulthood.  Society for Behavioral Neuroendocrinology Conference, Monterey, CA.

Heather Williams

Nathaniel T. Wheelwright, Meredith B. Swett, Iris I. Levin, Donald E. Kroodsma, Corey R. Freeman-Gallant, and Heather Williams (2008)  The influence of different tutor types on song learning in a natural bird population.  Animal Behaviour, 75, 1479-1493.


Betty Zimmerberg

Zimmerberg, B., Van Kempen, T.A., Martinez, A.R*. & Brunelli, S.A. (November, 2007).  Epigenetic effects of enriched environment housing on spatial learning deficits and hippocampal BDNF in high anxiety rats bred for an infantile vocalization phenotype.  Presented at the Society for Neuroscience annual meeting, San Diego, CA.

Pritchard, L.M., Van Kempen, T.A., Williams, H., Zimmerberg, B.  (November, 2007).  A laboratory exercise for a college-level, introductory neuroscience course demonstrating effects of housing environment on anxiety and psychostimulant sensitivity.  Presented at the Society for Neuroscience annual meeting, San Diego, CA.

Pritchard, L.M., Van Kempen, T.A.,  Zimmerberg, B.  (November, 2007).  Behavioral effects of repeated handling differ in rats reared in social isolation and environmental enrichment.  Presented at the Society for Neuroscience annual meeting, San Diego, CA.


Steve Zottoli

Greenwood, A.K., Peichel, K. and Zottoli, S. (2008) Does fast-start circuitry contribute to pufferfish inflation?   JBJC abstract.

Zottoli, S.J., Cioni, C. and Seyfarth, E.-A. (2008) Reticulospinal neurons in anamniotic vertebrates: A celebration of Alberto Stefanelli’s contributions to comparative neuroscience.    54th Convegno Gruppo Embriologico Italiano, Roma 4-7 giugno.



APPENDIX IV: NEUROSCIENCE FACULTY ACCOMPLISHMENTS

Professor Professor Lara Hutson

Lara Hutson was on leave all year, maternity leave during the fall and AP leave in the spring.  During this time, she continued her research on how small heat shock proteins (sHSPs) regulate zebrafish development and protect the developing embryo from the effects of environmental stressors.  Senior Essel Fellow, Martha Marvin, collaborated on this research, completing the work of characterizing the entire family of zebrafish small heat shock proteins.  The results of this work were published in the journal Developmental Dynamics (Marvin et al., 2008).  Kimberly Elicker ’09, Tahsin Khan ’10, and Nick Manice ‘09 also worked in the lab this year.  Kimberly and Tahsin studied the roles of Hsp27 and HspB8 during development of motor axon projections, and Tahsin began to develop transgenic zebrafish models for Charcot-Marie-Tooth Disease and Distal Hereditary Motor Neuropathy, both of which can be caused by mutations in small heat shock proteins.  Nick played an essential role in the lab assisting others with microinjections and tissue sectioning.  Tahsin and Kimberly will continue their work in the lab during the summer of 2008, and we will be joined by two new students, Beth Links ’09 and Clare Malone ’09.

Professor Noah Sandstrom

Noah Sandstrom continued his studies examining the neuroprotective effects of ovarian hormones in rodent models of global ischemia.  This work was conducted with seniors Gordon Crabtree ’08 and Erika Williams ’08.  Gordon and Prof. Sandstrom explored the neuroanatomical sites at which estradiol acts to protect hippocampal neurons against global ischemia-induced cell death in rats.  Erika and Prof. Sandstrom developed a mouse model of transient global ischemia and conducted several parametric studies of this model.  In addition, they have developed a colony of estrogen receptor knockout mice that will be used to explore the cellular mechanisms underlying estradiol-mediated neuroprotection.  Katie Jordon ’09 will continue this work in the coming year.

Magali Rowan ’07 and Erika Williams ’08 were coauthors of a presentation with Professor Sandstrom at the annual meeting of the Society for Neuroscience held in San Diego, CA.  In addition, research conducted as part of Sarah Chuzi’s ’07 senior thesis was presented at the annual meeting of the Society for Behavioral Neuroendocrinology in Monterey, CA.


Professor Paul Solomon

Paul Solomon continued to teach in the areas of neuropsychology and behavioral neuroscience.  He was responsible for the Clinical Neuroscience course taught this spring.  He also continued his work on the evaluation of pharmacological treatments for Alzheimer's disease.  This work was carried out in collaboration with several Williams neuroscience concentrators, including Carolyn Skudder and Nancy Haff, who worked on projects at The Memory Clinic in Bennington, VT.  Dr. Solomon's work continues to be supported by grants from the National Institute on Aging as well as grants from the pharmaceutical industry.  During the past year, Dr Solomon lectured widely throughout United States, Europe and Canada on diagnosis and treatment of Alzheimer's disease.


Professor Heather Williams

This year, Heather Williams' laboratory focused its attention on two primary issues: the basis of “syntax” in bird songs, and the long-term patterns of song learning and organization in the wild.  Two honors students, Mildred Duvet ’08 and Charles Upton ’08, did their honors thesis work on the song syntax of zebra finches.  These laboratory birds normally sing a string of notes in a linear sequence, but some birds vary this structure more than others.  Mildred Duvet quantified the songs of many adult male zebra finches bred in aviaries selected for birds with high- and low-variability songs, and compared the variability in song syntax to a number of brain and physiological measures.  She found that variability in song syntax is not tightly correlated with the volumes of specific song nuclei or with the testosterone levels of the birds, and her work suggests that details of neuronal wiring rather than heritable brain traits are responsible for differences between birds in the variability of song syntax.  Charlie Upton approached the same general problem from another angle:  he examined features of syllables and related those to how often birds varied their syntax at specific points within the learned song.  He found that birds tend to alter their syntax most often at points defined by specific syllable types and locations within the string.  Together, Mildred and Charlie’s work suggest that syntax variation arises as a result of song learning rather than genetically determined brain features.

During the fall semester, Prof. Williams taught Animal Behavior.  This course can be slanted in many different ways as different instructors at different institutions may choose to emphasize evolutionary, ecological, or neuroscience-related approaches.  At Williams College, although we do not neglect evolution and ecology, the course (not surprisingly) considers the neural basis of many animal behaviors, including bat echolocation, bird song learning, frog mate choice, pair formation in voles, and the “personality traits” behind baboon aggression.

Professor Williams was on sabbatical in the spring, and spent almost three weeks of that time alone on an island in the Bay of Fundy recording, capturing, banding, measuring, and mapping the territories of male Savannah sparrows as part of a long-term study.  The goal of this study is to use insights gained in the laboratory working with birds such as zebra finches to understand how individual learning of songs affects the structure of songs within a population – both in how they are spaced out in a given year and how they population changes over time.  The first paper about learning of songs in this species appeared this year (see below).

Three students – Danielle Perszyk ‘09, Daniel Tao ‘10 (both Essel Fellows), and Jon Cavanaugh – joined Prof. Williams in attending the annual Bird Song Conference held at the Rockefeller University.  This conference, which Williams co-founded, has proven to be highly successful and is now in its 12th year.

In work performed with Nathaniel Wheelwright and other co-authors, she published "The influence of different tutor types on song learning in a natural bird population" in the journal Animal Behaviour.

In February, Professor Williams served as a panelist for an N.I.H. study section (IFCN-5).


Professor Betty Zimmerberg

Professor Betty Zimmerberg continued her research on the effects of early social stress on the neural mechanisms underlying behavior.  Ashley Martinez ‘09 and Lindsay Moore ’09 conducted experiments in the summer of 2007 on the effects of neonatal isolation on olfactory associative learning and BDNF in the olfactory bulbs and hippocampi of week-old rats.  In the fall, Ashley also conducted an independent study project looking at the effects of gestational treatment with the neurosteroid allopregnenolone on maternal behavior in a line of rats genetically bred for increased anxiety.  During Winter Study, Cate Cho ’10 and Jim Dunshee ’09 conducted independent research on vocalization behavior of adult rats in lines bred for high and low rates of vocalization after brief maternal separation.  Tracey Van Kempen ’05, the Essel Foundation post-baccalaureate fellow, was an admirable technical assistant in all of these studies, and Shivon Robinson ’11 was an excellent research assistant.  Professor Zimmerberg also sponsored off-campus lab internships by Nicole Sanders ’08, Douglas Washington ’08 and Michael Kirwan ’08.

Zimmerberg taught the Program’s senior seminar in the fall of 2007, focusing on novel neurotransmitters and retrograde messengers in synaptic and behavioral function.  She also sponsored an independent study by Lindsay Moore ’09 on Neuroethics.  In the spring, she taught her seminar "Image, Imaging and Imagination: The Brain and Visual Arts".  This class examined topics at the intersection of psychology, neuroscience and art such as retinal neurobiology, cortical organization and perception of color and form, how visual artists have used or challenged perceptual cues in their work, and how the brain perceives faces and how that influences appreciation of portraiture.  They also considered the influence of neurological and psychological disorders in artists on their work.  In addition to regular seminar discussions, this class met several times in area museums and took a field trip to a New York City gallery. 

Zimmerberg served on the NSF’s SOMAS Grants Review Board and was an ad hoc reviewer for Behavioral Neuroscience and Neuroendocrinology grants for the NSF.  Other professional activities included serving on the editorial board of Developmental Psychobiology and reviewing manuscripts for Developmental Psychobiology; Behavioral Neuroscience; Behavioural Brain Research; Pharmacology, Biochemistry and Behavior; Alcohol; Progress in Neuro-Psychopharmacology & Biological Psychiatry; Biological Psychiatry.  In May, Zimmerberg attended Tufts University Conference on Emerging Trends in Behavioral, Affective, Social, and Cognitive (BASC) Neurosciences on the Cognitive Neuroscience of Visual Knowledge: “Where Vision Meets Memory”.   


Professor Steve Zottoli

Steve Zottoli began his first year as Chair of the Biology Department.  During the fall semester, Zottoli team-taught Introduction to Neuroscience with Noah Sandstrom in Psychology.  In the spring, he taught an upper level Neurobiology course which had been bracketed for a number of years.  He mentored two Honors Students, Tina Wong ’08 and Jarrad Wood ’08.  A long-term goal of the Zottoli laboratory is to understand the neuronal basis of behavior and the recovery of behavior after spinal cord injury.  Tina Wong studied physiological properties of supramedullary neurons in the pufferfish and successfully recorded from these neurons in a free-swimming fish.  Jarrad Wood studied the response of central nervous system glial cells and blood-born reactive cells to a minimal wound of a single axon (the Mauthner axon) in the brain of the goldfish.  Zottoli continues to spend summers at the Marine Biological Laboratory in Woods Hole, MA where he conducts research and is a faculty member in the SPINES (Summer Program in Neuroscience, Ethics and Survival) course.  Tina Wong ’08 spent the summer at the MBL with Zottoli studying the function of supramedullary neurons in fishes.

Zottoli is a Life Trustee of The Grass Foundation, a not-for-profit philanthropic organization that funds various programs in neuroscience.  He continues collaborative research projects with Melina Hale at the University of Chicago, Ed Gilland and Hans Straka at the MBL, Don Faber at Albert Einstein College of Medicine and Joe Fetcho at Cornell University.



APPENDIX V: OUTSIDE SOURCES OF FUNDING


Professor Lara Hutson

National Eye Institute (NIH) R03 Small grants for pilot research for: “Small heat shock proteins and retinotectal development,” 8/1/04-7/31/07 with no-cost extension through 7/31/08 ($373,868).

NIH R15 “Motor axon development in a zebrafish model of Charcot-Marie-Tooth disease,” 8/1/08-7/31/10 ($220,076)

Professor Noah Sandstrom

7/1/05 – 6/30/08.  “Estrogen and Cognition Following Ischemia.”  National Institutes of Health.  $213,007.  (1 R15 NS052911-01)

Professor Paul Solomon

Alzheimer's Disease Cooperative Study. "A Randomized, Double-Blind, Placebo-Controlled Trial of Valproate to attenuate the progression of Alzheimer's Disease." National Institute on Aging.

Principal Investigator --Sanofi-Synthabolab.  “A Dose-Ranging, Placebo-Controlled Study of SL65.0155-10 at the doses of 0.5 mg, 2 mg, and 8 mg for 12 Weeks in Patients with Mild-to-Moderate Alzheimer’s Disease.”

Principal Investigator – Wyeth Research.  “A 3-Month, Randomized, Double-Blind, Placebo-Controlled, Multi-Center, Safety, Tolerability, and Efficacy Study of 3 Doses of SRA-333 in Outpatient with Mild to Moderate Alzheimer’s Disease with Donepezil as Active Control.”

Principal Investigator – Eisai, Inc. "A 1-Year, Double-Blind, Randomized, Placebo-Controlled Study Of Rasagiline 1mg And 2mg Added to Aricept 10mg Daily in Patients with Mild to Moderate Dementia of the Alzheimer's Type."

Principal Investigator - Saegis, “A Multicenter, Randomized, Double-Blind, Placebo-Controlled, Phase II Study of Efficacy and Safety of SGS742 in Subjects with Mild to Moderate Alzheimer’s Disease.”

Principal Investigator - Sanofi-Synthelabo. “A Randomized, Multicenter, Double-Blind, Placebo-Controlled, 18-Month Study of the Efficacy of Xaliproden in Patients with Mild-to-Moderate Dementia of the Alzheimer’s Type.”

Principal Investigator - Elan Pharmaceuticals. “A Phase IIa, Multicenter, Randomized, Double-Blind, Placebo-Controlled, Multiple Ascending Dose, Safety, Tolerability, Pharmacokinetic, Pharmacodynamic, and Immunogenicity Trial of AAB-001 in Patients with Mild To Moderate Alzheimer’s Disease.”

Principal Investigator Voyager Pharmaceutical Corp, “A Double-Blind Placebo-Controlled Study Of Vp4896 For The Treatment Of Mild To Moderate Alzheimer’s Disease.” 

Principal Investigator - Myriad Pharmaceuticals. “Phase 3 Multicenter, Randomized, Double Blind, Placebo Controlled Study Of The Effect Of Daily Treatment With Mpc-7869 On Measures Of Cognition, Activities Of Daily Living And Global Function In Subjects With Mild Dementia Of The Alzheimer’s Type.” 

Principal Investigator - Sanofi Aventis, "A Fifty-Two-Week Multicenter Open-Label Study Evaluation The Long-Term Safety And Tolerability Of Saredutant In Adult And Elderly Patients With Major Depressive Disorder."


APPENDIX VI: ESSEL-SPONSERED COLLOQUIUM SPEAKERS

Magdalene Moran ‘96, Ph D, Hydra Biosciences
TRPs and TRPA1 Ion Channels in the Nervous System
October 17, 2007

Magdalene Moran is Senior Director of Biology at Hydra Biosciences, a biopharmaceutical company founded in 2002. Her research group focuses on drug discovery of small-molecule regulators for ion channels. TRPs (Transient Receptor Channels) are neuronal ion channels that relay information into and out of the cell. Specific TRP channels are involved in pain, hypertension and inflammation. Hydra Biosciences' first TRP channel blocker may be indicated for treatment of arthritis, chronic and acute pain. As the first in a novel class of pain relievers and anti-inflammatory drugs, it may avoid the side effects caused by opiates and NSAIDs (non-steroidal anti-inflammatory drugs). Additionally, she directs research on modulators of the CatSper (Cation Channel of Sperm) ion channels. CatSpers are found in the tail of mature sperm and are required for fertilization. Blockers of CatSper function are potential non-hormonal contraceptives.

 

Nathan Fox ‘70, PhD, University of Maryland, College Park
Behavioral inhibition: Linking Biology and Behavior
October 31, 2007

After graduating with Honor is Political Science from Williams College, Dr. Fox went on to study for his doctoral degree in Psychology and Social Relations at Harvard University.  Dr. Fox is currently the Director of the Child Development Lab at the Institute for Child Study in the Department of Human Development at the University of Maryland, College Park.  His research focuses on socio-emotional development in children, including temperament, development of emotion and emotion regulation, psychophysiology, and infant cognitive/social development.  Of particular interest to his lab is the observation and measurement of attention, memory, as well as emotion expression and social experience.  In his research, Dr. Fox links these psychological processes to neural activity through brain imaging methods such as EEG, ERP and functional neuroimaging.

 

Judy Willis ’71, M Ed, MD, RADteach.com
"Brain-Research Based Strategies for Teaching and Learning"
November 7-8, 2007

Dr. Willis '71 received her M.D. from the University of California at Los Angeles School of Medicine and her M.Ed. from the University of California at Santa Barbara.   After practicing as an adult and child neurologist for 15 years, she decided to pursue a career in education.  Since then, she has been teaching middle school and infusing her classroom practices with her expertise in neurology.  Her research focuses on RAD learning, an acronym that stands for the parts of the neural system that are particularly active in learning and memory: Reticular activating system, Amygdala, and Dopamine.  She also defines RAD as "reach and discover" for the less-scientifically inclined among her audience.  She is the author of a number of books, including "Research-Based Strategies to Ignite Student Learning: Insights from a Neurologist/Classroom Teacher" (2006),”Brain-Friendly Strategies for the Inclusion Classroom" (2007), and "How Your Child Learns Best: Insights from a Neurologist and Classroom Teacher" (upcoming, 2008).  Willis also writes extensively for professional educational journals and was honored as a 2007 Finalist for the Distinguished Achievement Award by the Association for Educational Publishers.

 

Peter Tyack, Ph D, Woods Hole Oceanographic Institute
"How Toothed Whales Echolocate to Find and Capture Prey"
November 30, 2007

Dr. Tyack is as Senior Scientist and Walter A and Hope Noyes Smith Chair in the Biology Department of the Woods Hole Oceanographic Institution.  His research focuses on the social behavior and acoustic communication in whales and dolphins as well as vocal learning and mimicry in their natural communication systems.  In particular, Dr. Tyack is investigating what the structure and function of whale songs can reveal about how whales and dolphins respond to human noise, as well as the social function of these songs among cetaceans.  Developing methods to identify which cetacean produces a sound within a social group of conspecifics along with new techniques to tag whales, locate their calls, and monitor vocal and social behavior of marine mammals is a crucial part of furthering this investigation.  He has been involved in the design, planning and fieldwork for a series of experiments investigating the possible impact on marine mammals of human-made sources of noise.

 

Chi-Bin Chien, Ph D, University of Utah School of Medicine
How the Zebrafish's Eye Connects to its Brain
December 4, 2007

After receiving his Ph.D. 1991 from the California Institute of Technology, Dr. Chien held post-doctoral fellowships at both the University of California San Diego and the Max Planck Institute for Developmental Biology in Germany prior to his current position as Associate Professor of Neurobiology and Anatomy at the University of Utah School of Medicine.  Dr. Chien’s research uses the zebrafish as a model system to understand how genetic and molecular mechanisms control cell behavior in vivo.  Many of the signaling molecules that are critical for development have now been identified: for instance, guidance ligands and receptors during axon and vascular guidance, and morphogens and transcription factors during embryonic patterning.  It is much less clear how these molecules function in cells during development, and furthermore how multiple signaling pathways are orchestrated as the embryo develops.

 

Bevil Conway, Ph D, Wellesley College, McLean Hospital, and Harvard Medical School
Constructing Color: Neural Mechanisms of Color Vision
February 14, 2008

Dr. Conway is a neuroscientist and artist, and is the Knafel Assistant Professor of Natural Science at Wellesley College.  His research examines the neural basis for visual behavior, with a particular focus on color vision.  Dr. Conway is particularly interested in investigating the relationship between visual processing and visual art -- what can art tell us about how the brain works?  Can neuroscience, vice versa, inform our understanding of visual art?



APPENDIX VII: YEAR 16 BUDGET AND EXPENDITURES

 

 

Budget

Expenditures

Undergraduate Summer Program

 

 

Student stipends ($360 per week x 124.6 weeks;
             spread among 13 students, some
             of whom spent less than 10 weeks)

44,200

44,856

Fringe benefits (mandated; 10%)

4,420

4,486

Research expenses ($4000 per faculty lab x 6 faculty)

24,000

24,000

Faculty summer salary ($4000 x 6 faculty members)

24,000

24,000

Fringe benefits (mandated; 3% or 10%,
                           depending on base salary)

720

991  

Essel Fellows Program

Senior Essel Fellow Salary

41,057

34,057

Fringe benefits (budgeted at 29%; actually 34%)

11,906

11,579

Junior Essel Fellow Salary

26,316

25,694

Fringe benefits (budgeted at 29%; actually 34%)

5,526

8,737

Research expenses for Senior Essel Fellow

4,000

4,000

Director’s compensation

4,000

4,000

Fringe benefits (mandated; 3%)

120

120

Student Travel Support

6,000

7,437

Neuroscience Conference

5,000

0*

2007-8 Total:

201,265

193,957

During 2007-8 academic year (the 2008 fiscal year), the Essel budget was underspent by $7,308. Substantially less was spent on the Senior Essel Fellow’s salary than budgeted because 20% of that salary was paid by Williams College to cover the Fellow’s teaching assignment; some of this savings was cancelled by the higher-than-budgeted fringe benefit rate.  In addition, the funds budgeted for the Essel Neuroscience Conference were not spent (the conference was held in 2006-7).  Student travel and housing was slightly overspent, as several students worked on projects off-campus.  We anticipate applying the unspent funds to the following fiscal year, as several fixed costs (for example, student stipends and fringe benefits, which are set by the college) have risen more rapidly than anticipated in the original budget.


APPENDIX VIII: UPPER LEVEL NEUROSCIENCE CLASSES AND EMPIRICAL PROJECTS

BIOL 204 - Animal Behavior – Heather Williams

Making sense of what we see while watching animals closely is both an enthralling pastime and a discipline that draws on many aspects of biology.  Explanations can be found on many levels: evolutionary theory tells us why certain patterns have come to exist, molecular biology can help us understand how those patterns are implemented, neuroscience gives insights as to how the world appears to the behaving animal, endocrinology provides information on how suites of behaviors are regulated.  The first part of the course focuses upon how descriptive studies provide the basis for formulating questions about behavior as well as the statistical methods used to evaluate the answers to these questions.  We then consider the behavior of individuals, both as it is mediated by biological mechanisms and as it appears from an evolutionary perspective.  The second half of the course is primarily concerned with the behaviors of groups of animals from a wide variety of vertebrate and invertebrate species, concentrating upon the stimuli, responses, and internal mechanisms that maintain social systems and on the selection pressures that drive animals toward a particular social system.

 

BIOL 304 – Neurobiology –Steve Zottoli

This course is concerned with understanding the biology of the nervous system, focusing primarily on the cellular bases of neuronal function.  Lectures will cover such topics as nerve resting and action potentials, ion channels, neurotransmitters and synapses, and the neural correlates of behavior in organisms with simple nervous systems.  Reading original research papers and discussing them constitutes an important part of the course.  Some of the topics that may be covered include: transmitter release mechanisms, ion permeation through channels, plasticity in the nervous system, and various clinical disorders.  Laboratories are designed to introduce the students to modern techniques in neurobiology including extracellular and intracellular recording, histochemistry, and immunohistochemistry.    

 

INTR 223 - Image, Imaging and Imagining: The Brain and Visual Arts – Betty Zimmerberg

The brain interprets the visual world and generates cognitive and emotional responses to what the eyes see.  It is also responsible for creating visual objects.  This course first examines how we see and how our brains organize and perceive what we see.  In that context, we will investigate how visual artists have used or challenged perceptual cues in their work.  We then will study Gestalt perceptual laws and illusions, and see how they have been used in works of art.  We will also consider the influence of "disturbed" brain function of artists on their work (for example, autism, schizophrenia, and epilepsy).  In the second unit, we will discuss the history and use of neuroimaging (brain scans), and the questions posed by Dumit's ethnography of neuroimagers, "Picturing Personhood," for example, how neuroimages are used in public discussions of mental illness, violence, and addiction.  We will also examine neuroimaging studies questioning whether the brains of visual artists are lateralized or specialized differently from non-artists.  In the final unit, we will explore how visual artists are using brain images in their artwork, and how they have portrayed brain syndromes and mental states.  The course will culminate with the development of an exhibit.                                                            

PSYC 315 - Hormones and Behavior – Noah Sandstrom

In all animals, hormones are essential for the coordination of basic functions such as development and reproduction.  This course studies the dynamic relationship between hormones and behavior.  We will review the mechanisms by which hormones act in the nervous system.  We will also investigate the complex interactions between hormones and behavior.  Specific topics to be examined include: sexual differentiation; reproductive and parental behaviors; stress; aggression; and learning and memory.  Students will critically review data from both human and animal studies.  All students will design and conduct an empirical research project.

 

PSYC 316 - Clinical Neuroscience – Paul Solomon

Diagnosing and treating neurological diseases is the final frontier of medicine.  Recent advances in neuroscience have had a profound impact on the understanding of diseases that affect cognition, behavior, and emotion.  This course provides an in-depth analysis of the relationship between brain dysfunction and disease state.  We will focus on neurodegenerative disorders including Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease.  We will consider diagnosis of disease, treatment strategies, as well as social and ethical issues.  The course provides students with the opportunity to present material based upon: (1) review of published literature, (2) analysis of case histories, and (3) observations of diagnosis and treatment of patients both live and on videotape.  All students will design and conduct an empirical research project.  Format: Empirical Lab Course.  Evaluation will be based on position papers, class participation, and research project report.

 

NSCI 401 - Topics in Neuroscience - Betty Zimmerberg, Martha Marvin

To create a culminating senior experience in which previous course work in specific areas in the Neuroscience Program can be brought to bear in a synthetic, interdisciplinary approach to understanding complex problems.  The specific goals for students in this seminar are (1) to evaluate original research and critically examine the experimental evidence for theoretical issues, and (2) to gain an understanding of this discipline through group work, and oral presentations.  Topics and instructional formats will vary somewhat from year to year, but in all cases, the course will emphasize an integrative approach in which students will be asked to consider topics from a range of perspectives including molecular, cellular, systems, behavioral and clinical neuroscience.  Previous topics have included memory, autism, depression, alcoholism, language development, and stress.

 


 

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